Journal: STAR Protocols
Article Title: Protocol to study adult neurogenesis in fresh-frozen human hippocampal tissue using an immunofluorescence quantitative approach
doi: 10.1016/j.xpro.2025.104344
Figure Lengend Snippet: Overview of expected outcomes (A) Schematic representation of distinct cell populations related to adult hippocampal neurogenesis (AHN). (B–H) Expected staining with anti- (B) vimentin, (C) sex determining region Y-box 2 (Sox2), (D) phospho-histone 3 (PH3), (E) HuC-HuD, (F) Doublecortin (DCX), (G) polysialylated-neural cell adhesion molecule (PSA-NCAM), and (H) calbindin (CB) antibodies. (I) Schematic representation of the components of the human hippocampal neurogenic niche. (J–M) Expected staining with anti- (J) S100 calcium-binding protein β (S100β), (K) ionized calcium-binding adaptor molecule 1 (Iba1), (L) phosphorylated γH2A.X (γH2A.X) antibodies, and (M) Ulex Europaeus Agglutinin-I (UEA-1). Yellow scale bar: 20 μm. White scale bar: 10 μm. Yellow triangles indicate positive cells. White triangles highlight the morphology of cells labelled for each marker.
Article Snippet: Goat polyclonal anti-SRY (sex-determining region Y)-box 2 (Sox2) (1:500) , R and D Systems , Cat# AF2018; RRID: AB_355110.
Techniques: Staining, Binding Assay, Marker